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KMID : 0380020120270040227
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Korean Journal of Biotechnology and Bioengineering
2012 Volume.27 No. 4 p.227 ~ p.231
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Cytotoxic Effect of Zostera asiatica on Growth of Human Cancer Cells
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Hong Joo-Wan
Jung Myung-Eun
Lee Jung-Im
Kim Ho-Jun
Chang Jae-Soo
Seo Young-Wan
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Abstract
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Whole plants of Zostera asiatica were extracted twice with acetone/methylene chloride (A+M) and methanol (MeOH) in turn. The combined crude extracts were evaporated in vacuo and then the residue was partitioned between water and methylene chloride. The aqueous layer was fractionated into H2O and n-butanol and then the organic layer was also fractionated into 85% aq. MeOH and n-hexane, successively. The crude extracts and their solvent fractions were evaluated for their inhibitory effect on growth of human cancer cells AGS, HT-29, MCF-7, and HT-1080 cells by MTT reduction assay. Among samples tested, 85% aq. MeOH and n-hexane fractions showed strong cytotoxic effect against AGS, HT-29, and MCF-7 cells. On the other hand, for HT-1080 cell, 85% aq. MeOH fraction exhibited the strongest cytotoxic effect.
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KEYWORD
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Zostera asiatica, human cancer cells, cytotoxic effect, MTT assay
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